TNF-α responsive DNA star trigon formation from four hairpin probes and the analytical application
Cheng, Wenbo1,2,3; Yan, Wei1; Miao, Peng2,3
2017
发表期刊Science China Chemistry
ISSN1674-7291
卷号60期号:3页码:405-409
摘要Tumor necrosis factor-alpha (TNF-α) is a type of critical pro-inflammatory cytokines, which participates in numerous cellular signal pathways and is regarded as a critical protein biomarker for inflammatory based diseases. In this contribution, we have developed a strategy to fabricate multiple DNA star trigon structures with fluorescence signals from four hairpin probes which are detonated by a single molecule of TNF-α. This process causes significant enhancement of fluorescence and a sensitive and selective biosensor for TNF-α assay is constructed. This method is able to achieve the limit of detection (LOD) at 5 pg/mL (0.285 pM). Moreover, some other advantages such as fast response, high selectivity and convenient operation promise the potential use of this method for TNF-α measurement in point of care testing application. Upon further development, this strategy can also be converted to detect other analytes such as small molecules, nucleic acids and other proteins. © 2017, Science China Press and Springer-Verlag Berlin Heidelberg.
关键词Bioassay - Biosensors - Fluorescence - Molecules - Nucleic acids - Probes - Proteins - Stars
收录类别SCI ; Ei
语种英语
文献类型期刊论文
条目标识符http://ir.ioe.ac.cn/handle/181551/8888
专题微细加工光学技术国家重点实验室(开放室)
作者单位1.State Key Laboratory of Optical Technologies on Nano-Fabrication and Micro-Engineering, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu; 610209, China;
2.CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou; 215163, China;
3.University of Chinese Academy of Sciences, Beijing; 100049, China
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Cheng, Wenbo,Yan, Wei,Miao, Peng. TNF-α responsive DNA star trigon formation from four hairpin probes and the analytical application[J]. Science China Chemistry,2017,60(3):405-409.
APA Cheng, Wenbo,Yan, Wei,&Miao, Peng.(2017).TNF-α responsive DNA star trigon formation from four hairpin probes and the analytical application.Science China Chemistry,60(3),405-409.
MLA Cheng, Wenbo,et al."TNF-α responsive DNA star trigon formation from four hairpin probes and the analytical application".Science China Chemistry 60.3(2017):405-409.
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