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题名:
In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy
作者: Wang, Zhibin1,2,3; Wei, Lin1,2; He, Yi1,2; Li, Xiqi1,2; Shi, Guohua1,2; Zhang, Yudong1,2
出版日期: 2014
会议名称: Proceedings of SPIE: Twelfth International Conference on Photonics and Imaging in Biology and Medicine, PIBM 2014
会议日期: 2014
学科分类: Confocal microscopy - Fluorescence - Fluorescence microscopy - Gradient methods - Photonics - Stochastic systems
DOI: 10.1117/12.2068823
通讯作者: Wang, Zhibin
中文摘要: Adaptive optics is implemented in a confocal scanning fluorescence microscope with wavefront sensorless scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. In vivo vascular imaging of mice ear is completed and the results revealed the improved signal and resolution leading to in substantially enhanced image contrast with aberration correction which allowed us to detect clearer vasculature structures. © 2014 SPIE.
英文摘要: Adaptive optics is implemented in a confocal scanning fluorescence microscope with wavefront sensorless scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. In vivo vascular imaging of mice ear is completed and the results revealed the improved signal and resolution leading to in substantially enhanced image contrast with aberration correction which allowed us to detect clearer vasculature structures. © 2014 SPIE.
收录类别: Ei
语种: 英语
卷号: 9230
ISSN号: 16057422
文章类型: 会议论文
页码: 92301W
Citation statistics:
内容类型: 会议论文
URI标识: http://ir.ioe.ac.cn/handle/181551/7824
Appears in Collections:自适应光学技术研究室(八室)_会议论文

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作者单位: 1. Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu, China
2. Laboratory on Adaptive Optics, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu, China
3. Graduate School of Chinese, Academy of Sciences, Beijing, China

Recommended Citation:
Wang, Zhibin,Wei, Lin,He, Yi,et al. In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy[C]. 见:Proceedings of SPIE: Twelfth International Conference on Photonics and Imaging in Biology and Medicine, PIBM 2014. 2014.
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