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In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy
Wang, Zhibin1,2,3; Wei, Lin1,2; He, Yi1,2; Li, Xiqi1,2; Shi, Guohua1,2; Zhang, Yudong1,2
Volume9230
Pages92301W
2014
Language英语
ISSN16057422
DOI10.1117/12.2068823
Indexed ByEi
Subtype会议论文
AbstractAdaptive optics is implemented in a confocal scanning fluorescence microscope with wavefront sensorless scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. In vivo vascular imaging of mice ear is completed and the results revealed the improved signal and resolution leading to in substantially enhanced image contrast with aberration correction which allowed us to detect clearer vasculature structures. © 2014 SPIE.; Adaptive optics is implemented in a confocal scanning fluorescence microscope with wavefront sensorless scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. In vivo vascular imaging of mice ear is completed and the results revealed the improved signal and resolution leading to in substantially enhanced image contrast with aberration correction which allowed us to detect clearer vasculature structures. © 2014 SPIE.
Conference NameProceedings of SPIE: Twelfth International Conference on Photonics and Imaging in Biology and Medicine, PIBM 2014
Conference Date2014
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type会议论文
Identifierhttp://ir.ioe.ac.cn/handle/181551/7824
Collection自适应光学技术研究室(八室)
Corresponding AuthorWang, Zhibin
Affiliation1. Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu, China
2. Laboratory on Adaptive Optics, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu, China
3. Graduate School of Chinese, Academy of Sciences, Beijing, China
Recommended Citation
GB/T 7714
Wang, Zhibin,Wei, Lin,He, Yi,et al. In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy[C],2014:92301W.
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