| In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy | |
| Wang, Zhibin1,2,3; Wei, Lin1,2; He, Yi1,2; Li, Xiqi1,2; Shi, Guohua1,2; Zhang, Yudong1,2 | |
| Volume | 9230 |
| Pages | 92301W |
| 2014 | |
| Language | 英语 |
| ISSN | 16057422 |
| DOI | 10.1117/12.2068823 |
| Indexed By | Ei |
| Subtype | 会议论文 |
| Abstract | Adaptive optics is implemented in a confocal scanning fluorescence microscope with wavefront sensorless scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. In vivo vascular imaging of mice ear is completed and the results revealed the improved signal and resolution leading to in substantially enhanced image contrast with aberration correction which allowed us to detect clearer vasculature structures. © 2014 SPIE.; Adaptive optics is implemented in a confocal scanning fluorescence microscope with wavefront sensorless scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. In vivo vascular imaging of mice ear is completed and the results revealed the improved signal and resolution leading to in substantially enhanced image contrast with aberration correction which allowed us to detect clearer vasculature structures. © 2014 SPIE. |
| Conference Name | Proceedings of SPIE: Twelfth International Conference on Photonics and Imaging in Biology and Medicine, PIBM 2014 |
| Conference Date | 2014 |
| Citation statistics | |
| Document Type | 会议论文 |
| Identifier | http://ir.ioe.ac.cn/handle/181551/7824 |
| Collection | 自适应光学技术研究室（八室） |
| Corresponding Author | Wang, Zhibin |
| Affiliation | 1. Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu, China 2. Laboratory on Adaptive Optics, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu, China 3. Graduate School of Chinese, Academy of Sciences, Beijing, China |
| Recommended Citation GB/T 7714 | Wang, Zhibin,Wei, Lin,He, Yi,et al. In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy[C],2014:92301W. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 2014-2170.pdf（480KB） | 会议论文 | 开放获取 | CC BY-NC-SA | Application Full Text | ||
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