| 题名: | In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy |
| 作者: | Wang, Zhibin1,2,3; Wei, Lin1,2; He, Yi1,2; Li, Xiqi1,2; Shi, Guohua1,2; Zhang, Yudong1,2
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| 出版日期: | 2014
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| 会议名称: | Proceedings of SPIE: Twelfth International Conference on Photonics and Imaging in Biology and Medicine, PIBM 2014
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| 会议日期: | 2014
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| 学科分类: | Confocal microscopy - Fluorescence - Fluorescence microscopy - Gradient methods - Photonics - Stochastic systems
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| DOI: | 10.1117/12.2068823
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| 通讯作者: | Wang, Zhibin
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| 中文摘要: | Adaptive optics is implemented in a confocal scanning fluorescence microscope with wavefront sensorless scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. In vivo vascular imaging of mice ear is completed and the results revealed the improved signal and resolution leading to in substantially enhanced image contrast with aberration correction which allowed us to detect clearer vasculature structures. © 2014 SPIE. |
| 英文摘要: | Adaptive optics is implemented in a confocal scanning fluorescence microscope with wavefront sensorless scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. In vivo vascular imaging of mice ear is completed and the results revealed the improved signal and resolution leading to in substantially enhanced image contrast with aberration correction which allowed us to detect clearer vasculature structures. © 2014 SPIE. |
| 收录类别: | Ei
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| 语种: | 英语
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| 卷号: | 9230
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| ISSN号: | 16057422
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| 文章类型: | 会议论文
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| 页码: | 92301W
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| Citation statistics: |
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| 内容类型: | 会议论文
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| URI标识: | http://ir.ioe.ac.cn/handle/181551/7824
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| Appears in Collections: | 自适应光学技术研究室（八室）_会议论文
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| 2014-2170.pdf（480KB） | 会议论文 | -- | 限制开放 | | View
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作者单位: | 1. Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu, China
2. Laboratory on Adaptive Optics, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu, China
3. Graduate School of Chinese, Academy of Sciences, Beijing, China
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| Recommended Citation: | |
Wang, Zhibin,Wei, Lin,He, Yi,et al. In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy[C]. 见:Proceedings of SPIE: Twelfth International Conference on Photonics and Imaging in Biology and Medicine, PIBM 2014. 2014.
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