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题名:
Formation and stimuli-directed migration of D. discoideum slugs in microchips
作者: Kim, Jinho1; Olsen, Timothy1; Zhuang, Xuye2; Luo, Ji2; Yao, Jun3; Stojanovic, Milan3; Lin, Qiao1
刊名: Journal of Medical and Biological Engineering
出版日期: 2013
卷号: 33, 期号:3, 页码:263-268
学科分类: Fluidic devices - Microactuators - Microorganisms - Optical fibers
DOI: 10.5405/jmbe.1322
通讯作者: Lin, Q. (qlin@columbia.edu)
文章类型: 期刊论文
中文摘要: This paper presents a microfluidic device that geometrically constrains the development of individual Dictyostelium discoideum cells into multicellular organisms (slugs). A microchip for the stimuli-directed migration of slugs is also presented. To demonstrate the formation of slugs in a predetermined shape, a microchip is designed to confine the slugs in the vertical direction. In the microchip, sufficient oxygen is supplied to the cells via a membrane, allowing the formation and adaptation of slugs to the shape of the channel. In addition, the manipulation of slug migration direction in a microchip via external stimuli, such as light and temperature gradients, that induce phototaxis and thermotaxis of slugs, respectively, is demonstrated. To direct slug migration with external stimuli, an optical fiber is used for phototaxis and an electrical wire heater is used for thermotaxis. Experimental results show slug formation in a predefined geometry in the microchip, suggesting that this chip is potentially useful for understanding the relationship between the shape and function of cells or tissue. The controlled migration of slugs demonstrated in the microchips can potentially be employed in biologically based microactuators or microrobots.
英文摘要: This paper presents a microfluidic device that geometrically constrains the development of individual Dictyostelium discoideum cells into multicellular organisms (slugs). A microchip for the stimuli-directed migration of slugs is also presented. To demonstrate the formation of slugs in a predetermined shape, a microchip is designed to confine the slugs in the vertical direction. In the microchip, sufficient oxygen is supplied to the cells via a membrane, allowing the formation and adaptation of slugs to the shape of the channel. In addition, the manipulation of slug migration direction in a microchip via external stimuli, such as light and temperature gradients, that induce phototaxis and thermotaxis of slugs, respectively, is demonstrated. To direct slug migration with external stimuli, an optical fiber is used for phototaxis and an electrical wire heater is used for thermotaxis. Experimental results show slug formation in a predefined geometry in the microchip, suggesting that this chip is potentially useful for understanding the relationship between the shape and function of cells or tissue. The controlled migration of slugs demonstrated in the microchips can potentially be employed in biologically based microactuators or microrobots.
收录类别: SCI ; Ei
项目资助者: National Science Foundation [CBET-0854030] ; National Institutes of Health [RR025816-02, CA147925-01] ; K.C. Wong Education Foundation, Hong Kong
语种: 英语
WOS记录号: WOS:000322734800004
ISSN号: 16090985
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ioe.ac.cn/handle/181551/6840
Appears in Collections:微细加工光学技术国家重点实验室(开放室)_期刊论文

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作者单位: 1. Department of Mechanical Engineering, Columbia University, New York, NY 10027, United States
2. Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China
3. Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY 10032, United States

Recommended Citation:
Kim, Jinho,Olsen, Timothy,Zhuang, Xuye,et al. Formation and stimuli-directed migration of D. discoideum slugs in microchips[J]. Journal of Medical and Biological Engineering,2013,33(3):263-268.
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