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Formation and stimuli-directed migration of D. discoideum slugs in microchips
Kim, Jinho1; Olsen, Timothy1; Zhuang, Xuye2; Luo, Ji2; Yao, Jun3; Stojanovic, Milan3; Lin, Qiao1; Lin, Q. (qlin@columbia.edu)
Source PublicationJournal of Medical and Biological Engineering
Volume33Issue:3Pages:263-268
2013
Language英语
ISSN16090985
DOI10.5405/jmbe.1322
Indexed BySCI ; Ei
WOS IDWOS:000322734800004
Subtype期刊论文
AbstractThis paper presents a microfluidic device that geometrically constrains the development of individual Dictyostelium discoideum cells into multicellular organisms (slugs). A microchip for the stimuli-directed migration of slugs is also presented. To demonstrate the formation of slugs in a predetermined shape, a microchip is designed to confine the slugs in the vertical direction. In the microchip, sufficient oxygen is supplied to the cells via a membrane, allowing the formation and adaptation of slugs to the shape of the channel. In addition, the manipulation of slug migration direction in a microchip via external stimuli, such as light and temperature gradients, that induce phototaxis and thermotaxis of slugs, respectively, is demonstrated. To direct slug migration with external stimuli, an optical fiber is used for phototaxis and an electrical wire heater is used for thermotaxis. Experimental results show slug formation in a predefined geometry in the microchip, suggesting that this chip is potentially useful for understanding the relationship between the shape and function of cells or tissue. The controlled migration of slugs demonstrated in the microchips can potentially be employed in biologically based microactuators or microrobots.; This paper presents a microfluidic device that geometrically constrains the development of individual Dictyostelium discoideum cells into multicellular organisms (slugs). A microchip for the stimuli-directed migration of slugs is also presented. To demonstrate the formation of slugs in a predetermined shape, a microchip is designed to confine the slugs in the vertical direction. In the microchip, sufficient oxygen is supplied to the cells via a membrane, allowing the formation and adaptation of slugs to the shape of the channel. In addition, the manipulation of slug migration direction in a microchip via external stimuli, such as light and temperature gradients, that induce phototaxis and thermotaxis of slugs, respectively, is demonstrated. To direct slug migration with external stimuli, an optical fiber is used for phototaxis and an electrical wire heater is used for thermotaxis. Experimental results show slug formation in a predefined geometry in the microchip, suggesting that this chip is potentially useful for understanding the relationship between the shape and function of cells or tissue. The controlled migration of slugs demonstrated in the microchips can potentially be employed in biologically based microactuators or microrobots.
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Document Type期刊论文
Identifierhttp://ir.ioe.ac.cn/handle/181551/6840
Collection微细加工光学技术国家重点实验室(开放室)
Corresponding AuthorLin, Q. (qlin@columbia.edu)
Affiliation1. Department of Mechanical Engineering, Columbia University, New York, NY 10027, United States
2. Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China
3. Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY 10032, United States
Recommended Citation
GB/T 7714
Kim, Jinho,Olsen, Timothy,Zhuang, Xuye,et al. Formation and stimuli-directed migration of D. discoideum slugs in microchips[J]. Journal of Medical and Biological Engineering,2013,33(3):263-268.
APA Kim, Jinho.,Olsen, Timothy.,Zhuang, Xuye.,Luo, Ji.,Yao, Jun.,...&Lin, Q. .(2013).Formation and stimuli-directed migration of D. discoideum slugs in microchips.Journal of Medical and Biological Engineering,33(3),263-268.
MLA Kim, Jinho,et al."Formation and stimuli-directed migration of D. discoideum slugs in microchips".Journal of Medical and Biological Engineering 33.3(2013):263-268.
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