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Detection of p53 gene mutation by using a novel biosensor based on localized surface plasmon resonance
Duan, R. Q.; Yuan, J. L.; Yang, H.; Luo, X. G.; Xi, M. R.; Xi, MR (reprint author), Sichuan Univ, Dept Gynecol & Obstet, W China Univ Hosp 2, Chengdu 610041, Peoples R China.
Source PublicationNEOPLASMA
Volume59Issue:3Pages:348-353
2012
Language英语
ISSN0028-2685
DOI10.4149/neo_2012_045
Indexed BySCI
WOS IDWOS:000301966700015
Subtype期刊论文
AbstractFew studies to date have reported on the development and application of a nanobiosensor based on localized surface plasmon resonance (LSPR) for detecting gene mutations. This study aimed to develop a novel LSPR biosensor used for detecting p53 mutation. Nanosphere lithography was used to fabricate the silver nanoparticles. The DNA probe was designed to recognize the target sequence and immobilized on the chip surface by a covalent-coupling method using amine-group ligands. Synthetic oligonucleotides or PCR products were amplified from genomic DNA taken from blood samples and hybridized with the immobilized probe. Wild-type and mutant p53 was detected by measuring shifts in peak of LSPR extinction spectra. The low detection limit of the sensor for target sequence was 10 nM, and detection occurred over a wide dynamic range (10 nM - 10 mu M). Importantly, the differences in measuring signal between wild-type and mismatched p53 DNA was significant, allowing for this sensor to effectively discriminate against single base mutations. In conclusion, we developed a biosensor with potential as a rapid, label-free, sensitive, and low-cost method for detecting p53 mutation. Our results suggest that such an LSPR-based biosensor provides an attractive alternative for clinical detection of genetic mutation.; Few studies to date have reported on the development and application of a nanobiosensor based on localized surface plasmon resonance (LSPR) for detecting gene mutations. This study aimed to develop a novel LSPR biosensor used for detecting p53 mutation. Nanosphere lithography was used to fabricate the silver nanoparticles. The DNA probe was designed to recognize the target sequence and immobilized on the chip surface by a covalent-coupling method using amine-group ligands. Synthetic oligonucleotides or PCR products were amplified from genomic DNA taken from blood samples and hybridized with the immobilized probe. Wild-type and mutant p53 was detected by measuring shifts in peak of LSPR extinction spectra. The low detection limit of the sensor for target sequence was 10 nM, and detection occurred over a wide dynamic range (10 nM - 10 mu M). Importantly, the differences in measuring signal between wild-type and mismatched p53 DNA was significant, allowing for this sensor to effectively discriminate against single base mutations. In conclusion, we developed a biosensor with potential as a rapid, label-free, sensitive, and low-cost method for detecting p53 mutation. Our results suggest that such an LSPR-based biosensor provides an attractive alternative for clinical detection of genetic mutation.
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Document Type期刊论文
Identifierhttp://ir.ioe.ac.cn/handle/181551/6809
Collection微细加工光学技术国家重点实验室(开放室)
Corresponding AuthorXi, MR (reprint author), Sichuan Univ, Dept Gynecol & Obstet, W China Univ Hosp 2, Chengdu 610041, Peoples R China.
Affiliation1.[Duan, R. Q.
2.Yuan, J. L.
3.Xi, M. R.] Sichuan Univ, Dept Gynecol & Obstet, W China Univ Hosp 2, Chengdu 610041, Peoples R China
4.[Yang, H.
5.Luo, X. G.] Chinese Acad Sci, State Key Lab Opt Technol Microfabricat, Inst Opt & Elect, Chengdu 610209, Peoples R China
Recommended Citation
GB/T 7714
Duan, R. Q.,Yuan, J. L.,Yang, H.,et al. Detection of p53 gene mutation by using a novel biosensor based on localized surface plasmon resonance[J]. NEOPLASMA,2012,59(3):348-353.
APA Duan, R. Q.,Yuan, J. L.,Yang, H.,Luo, X. G.,Xi, M. R.,&Xi, MR .(2012).Detection of p53 gene mutation by using a novel biosensor based on localized surface plasmon resonance.NEOPLASMA,59(3),348-353.
MLA Duan, R. Q.,et al."Detection of p53 gene mutation by using a novel biosensor based on localized surface plasmon resonance".NEOPLASMA 59.3(2012):348-353.
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