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题名:
In vivo imaging of mice auricle vessels using adaptive optical confocal fluorescence microscope
作者: He, Yi1,2; Wang, Zhibin1,2,4; Wang, Yuanyuan3,4; Wei, Ling1,2; Li, Xiqi1,2; Yang, Jinsheng1,2; Shi, Guohua1,2; Zhang, Yudong1,2
刊名: Chinese Optics Letters
出版日期: 2015
卷号: 13, 期号:11, 页码:111702
学科分类: Algorithms - Bandwidth - Fluorescence - Fluorescence microscopy - Gradient methods - Mammals - Stochastic systems - Underwater acoustics
DOI: 10.3788/COL201513.111702
通讯作者: He, Yi (heyi_job@126.com)
文章类型: 期刊论文
中文摘要: Facilitated with stochastic parallel gradient descent (SPGD) algorithm for wavefront sensorless correcting aberrations, an adaptive optics (AO) confocal fluorescence microscopy is developed and used to record fluorescent signals in vivo. Vessels of mice auricle at 80, 100 and 120 μm depth are obtained, and image contrast and fluorescence intensity are significantly improved with AO correction. The typical 10%-90% rise-time of the metric value measured is 5.0 s for a measured close-loop bandwidth of 0.2 Hz. Therefore, the AO confocal microscopy implemented with SPGD algorithm for robust AO corrections will be a powerful tool for study of vascular dynamics in future. © 2015 Chinese Optics Letters.
英文摘要: Facilitated with stochastic parallel gradient descent (SPGD) algorithm for wavefront sensorless correcting aberrations, an adaptive optics (AO) confocal fluorescence microscopy is developed and used to record fluorescent signals in vivo. Vessels of mice auricle at 80, 100 and 120 μm depth are obtained, and image contrast and fluorescence intensity are significantly improved with AO correction. The typical 10%-90% rise-time of the metric value measured is 5.0 s for a measured close-loop bandwidth of 0.2 Hz. Therefore, the AO confocal microscopy implemented with SPGD algorithm for robust AO corrections will be a powerful tool for study of vascular dynamics in future. © 2015 Chinese Optics Letters.
收录类别: SCI ; Ei
项目资助者: Outstanding Young Scientists of the Chinese Academy of Sciences ; National Instrumentation Program [2012YQ120080] ; Zhejiang Province Technology Program [2013C33170] ; National Science Foundation of China [61108082] ; Sichuan Youth Science & Technology Foundation [2013JQ0028] ; West Light Foundation of the Chinese Academy of Sciences
语种: 英语
WOS记录号: WOS:000364520000016
ISSN号: 1671-7694
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ioe.ac.cn/handle/181551/6416
Appears in Collections:自适应光学技术研究室(八室)_期刊论文

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作者单位: 1. The Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu, China
2. The Laboratory on Adaptive Optics, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu, China
3. School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical College, Wenzhou, China
4. University of Chinese Academy of Sciences, Beijing, China

Recommended Citation:
He, Yi,Wang, Zhibin,Wang, Yuanyuan,et al. In vivo imaging of mice auricle vessels using adaptive optical confocal fluorescence microscope[J]. Chinese Optics Letters,2015,13(11):111702.
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