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题名:
Aberration correction during real time in vivo imaging of bone marrow with sensorless adaptive optics confocal microscope
作者: Wang, Zhibin1,2,3; Wei, Dan4; Wei, Ling1,2; He, Yi1,2; Shi, Guohua1,2; Wei, Xunbin4; Zhang, Yudong1,2
刊名: Journal of Biomedical Optics
出版日期: 2014
卷号: 19, 期号:8, 页码:086009
学科分类: Adaptive optics - Fluorescence - Fluorescence microscopy - Gradient methods - Mammals
DOI: 10.1117/1.JBO.19.8.086009
通讯作者: Shi, G. (ioe_eye@126.com)
文章类型: 期刊论文
中文摘要: We have demonstrated adaptive correction of specimen-induced aberration during in vivo imaging of mouse bone marrow vasculature with confocal fluorescence microscopy. Adaptive optics system was completed with wavefront sensorless correction scheme based on stochastic parallel gradient descent algorithm. Using image sharpness as the optimization metric, aberration correction was performed based upon Zernike polynomial modes. The experimental results revealed the improved signal and resolution leading to a substantially enhanced image contrast with aberration correction. The image quality of vessels at 38-and 75-im depth increased three times and two times, respectively. The corrections allowed us to detect clearer bone marrow vasculature structures at greater contrast and improve the signal-to-noise ratio. © 2014 Society of Photo-Optical Instrumentation Engineers.
英文摘要: We have demonstrated adaptive correction of specimen-induced aberration during in vivo imaging of mouse bone marrow vasculature with confocal fluorescence microscopy. Adaptive optics system was completed with wavefront sensorless correction scheme based on stochastic parallel gradient descent algorithm. Using image sharpness as the optimization metric, aberration correction was performed based upon Zernike polynomial modes. The experimental results revealed the improved signal and resolution leading to a substantially enhanced image contrast with aberration correction. The image quality of vessels at 38-and 75-im depth increased three times and two times, respectively. The corrections allowed us to detect clearer bone marrow vasculature structures at greater contrast and improve the signal-to-noise ratio. © 2014 Society of Photo-Optical Instrumentation Engineers.
收录类别: SCI ; Ei
项目资助者: National Major Scientific Equipment program [2012YQ120080] ; National Science Foundation of China [61108082] ; Sichuan Youth Science and Technology Foundation [2013JQ0028] ; West Light Foundation of the Chinese Academy of Sciences
语种: 英语
WOS记录号: WOS:000341250300024
ISSN号: 10833668
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ioe.ac.cn/handle/181551/6378
Appears in Collections:自适应光学技术研究室(八室)_期刊论文

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作者单位: 1. Chinese Academy of Sciences, Key Laboratory on Adaptive Optics, Chengdu 610209, China
2. Chinese Academy of Sciences, Institute of Optics and Electronics, Laboratory on Adaptive Optics, Chengdu 610209, China
3. University of Chinese Academy of Sciences, Beijing 100039, China
4. Shanghai Jiao Tong University, School of Biomedical Engineering and Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China

Recommended Citation:
Wang, Zhibin,Wei, Dan,Wei, Ling,et al. Aberration correction during real time in vivo imaging of bone marrow with sensorless adaptive optics confocal microscope[J]. Journal of Biomedical Optics,2014,19(8):086009.
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