Nanopillar arrays have attracted considerable attention lately based on confinement effects and large surface to volume ratios. In this study, we demonstrated an efficient low-cost method for fabrication of large-area high aspect ratio nanopillars based on thiol-ene. Anodic aluminum oxide (AAO) template with ordered deep hole array was employed as the master mold. The low-viscosity and highly rigid UV-curable thiol-ene polymer was used as the structure material. The deep holes were filled with the liquid thiol-ene by overcoming the surface tension. Upon curing under UV irradiation, the thiol-ene formed a rigid cross-linked polymer network. The thiol-ene nanopillars were achieved after releasing the AAO mold in the corrosive solution, due to the corrosive-resistance of the cross-linked polymer. Based on the novel method, we fabricated thiol-ene nanopillar array with the diameter of 296 nm and aspect ratio of higher than 100:1. The experimental results demonstrated that the properties of thiol-ene polymer were excellent and the fabrication method was feasible. (C) 2015 Elsevier B.V. All rights reserved.