Facilitated with stochastic parallel gradient descent (SPGD) algorithm for wavefront sensorless correcting aberrations, an adaptive optics (AO) confocal fluorescence microscopy is developed and used to record fluorescent signals in vivo. Vessels of mice auricle at 80, 100 and 120 mu m depth are obtained, and image contrast and fluorescence intensity are significantly improved with AO correction. The typical 10%-90% rise-time of the metric value measured is 5.0 s for a measured close-loop bandwidth of 0.2 Hz. Therefore, the AO confocal microscopy implemented with SPGD algorithm for robust AO corrections will be a powerful tool for study of vascular dynamics in future.
Outstanding Young Scientists of the Chinese Academy of Sciences
; National Instrumentation Program(2012YQ120080)
; Zhejiang Province Technology Program(2013C33170)
; National Science Foundation of China(61108082)
; Sichuan Youth Science & Technology Foundation(2013JQ0028)
; West Light Foundation of the Chinese Academy of Sciences
1.Chinese Acad Sci, Key Lab Adapt Opt, Chengdu 610209, Peoples R China 2.Chinese Acad Sci, Inst Opt & Elect, Lab Adapt Opt, Chengdu 610209, Peoples R China 3.Wenzhou Med Coll, Sch Optometry, Wenzhou 325035, Peoples R China 4.Wenzhou Med Coll, Ophthalmol & Eye Hosp, Wenzhou 325035, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
He, Yi,Wang, Zhibin,Wang, Yuanyuan,et al. In vivo imaging of mice auricle vessels using adaptive optical confocal fluorescence microscope[J]. CHINESE OPTICS LETTERS,2015,13(11).