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题名:
Near infrared in vivo flow cytometry for tracking fluorescent circulating cells
作者: Suo, Yuanzhen1,2; Liu, Tao3; Xie, Chengying1,2; Wei, Dan1,2; Tan, Xu3; Wu, Liao3; Wang, Xiaoling1,2; He, Hao1,2; Shi, Guohua4,5; Wei, Xunbin1,2; Shi, Chunmeng3
刊名: CYTOMETRY PART A
出版日期: 2015-09-01
卷号: 87A, 期号:9, 页码:878-884
关键词: In vivo flow cytometry ; near infrared fluorescent dyes ; circulating cells ; stem cells
DOI: 10.1002/cyto.a.22711
文章类型: Article
英文摘要: The in vivo flow cytometry (IVFC) is now a powerful technique in biomedical research, especially for tracking specific cells in circulatory system. The current fluorescence-based IVFC is limited to visible spectrum, while near infrared (NIR) dyes have their advantages, such as deeper penetration, less absorption and less scattering for NIR fluorescence. Here, using an NIR in vivo flow cytometer with a 785 nm laser excitation, the measurement of fluorescent dye IR-780 labeled circulating cells is demonstrated. Representative peaks corresponding to NIR fluorescent circulating cells are detected and quantified. In addition, blood flow information, including the blood flow velocity and flow volume per unit time, is obtained. By simultaneous detection of IR-780 and enhanced green fluorescent protein (EGFP) signals from dual labeled cells, the IR-780 is shown to be a suitable fluorescent dye for multicolor detection by IVFC, including NIR. Thus, the IVFC is extended to the NIR range and shows potential application in biomedical research. (c) 2015 International Society for Advancement of Cytometry
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: HEPATOCELLULAR-CARCINOMA CELLS ; BONE-MARROW ; CANCER ; METASTASIS ; SKIN
收录类别: SCI
语种: 英语
WOS记录号: WOS:000360590500013
ISSN号: 1552-4922
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ioe.ac.cn/handle/181551/3702
Appears in Collections:光电技术研究所被WoS收录文章_期刊论文

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作者单位: 1.Shanghai Jiao Tong Univ, Med X Res Inst, Shanghai 200030, Peoples R China
2.Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200030, Peoples R China
3.Third Mil Med Univ, Chongqing Engn Res Ctr Nanomed, State Key Lab Trauma Burns & Combined Injury, Coll Prevent Med,Inst Combined Inju, Chongqing 400038, Peoples R China
4.Chinese Acad Sci, Key Lab Adapt Opt, Chengdu 610209, Peoples R China
5.Chinese Acad Sci, Inst Opt & Elect, Lab Adapt Opt, Chengdu 610209, Peoples R China

Recommended Citation:
Suo, Yuanzhen,Liu, Tao,Xie, Chengying,et al. Near infrared in vivo flow cytometry for tracking fluorescent circulating cells[J]. CYTOMETRY PART A,2015,87A(9):878-884.
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