IOE OpenIR  > 光电技术研究所被WoS收录文章
A reusable localized surface plasmon resonance biosensor for quantitative detection of serum squamous cell carcinoma antigen in cervical cancer patients based on silver nanoparticles array
Zhao, Qianying1; Duan, Ruiqi1; Yuan, Jialing1; Quan, Yi1; Yang, Huan2; Xi, Mingrong1
Source PublicationINTERNATIONAL JOURNAL OF NANOMEDICINE
Volume9Pages:1097-1104
2014
Language英语
Indexed BySCI
WOS IDWOS:000331702400001
SubtypeArticle
AbstractSquamous cell carcinoma antigen (SCCa), as a tumor biomarker, plays an important role in adjuvant diagnosis, treatment evaluation, and prognosis prediction for cervical cancer patients. Localized surface plasmon resonance (LSPR) technique based on noble metal nanoparticles bypasses the disadvantages of traditional testing strategies, in terms of free-labeling, short assay time, good sensitivity, and selectivity. Herein, we develop a novel and reusable LSPR biosensor for the detection of SCCa. First, a triangle-shaped silver nanoparticle array was fabricated using the nanosphere lithography method. Next, we investigated and verified the feasibility of amino coupling method using 11-mercaptoundecanoic acid (MUA) to form a functionalized chip surface with monoclonal anti-SCCa antibodies on the silver nanoparticles for distinct detection of SCCa. Different concentrations of SCCa were successfully tested in both buffer and human serum by the ultrasensitive and specific LSPR system, with a linear quantitative detection range of 0.1-1,000 pM under optimal conditions. With appropriate regeneration solution, for example 50 mM glycine-HCl (pH 2.0), the LSPR biosensor featured effective fabrication reproducibility, which reduced both production cost and testing time. Our study represents the first application of the LSPR biosensor in cervical cancer, and demonstrates that the rapid, simple, and reusable nanochip can serve as a potential alternative for clinical serological diagnosis of SCCa in cervical cancer patients.
KeywordLocalized Surface Plasmon Resonance Nanotechnology Biosensor Cervical Cancer Biomarker Squamous Cell Carcinoma Antigen
WOS KeywordTUMOR-ANTIGEN ; ASSAY ; RADIOIMMUNOASSAY ; HPV
WOS Research AreaScience & Technology - Other Topics ; Pharmacology & Pharmacy
WOS SubjectNanoscience & Nanotechnology ; Pharmacology & Pharmacy
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Document Type期刊论文
Identifierhttp://ir.ioe.ac.cn/handle/181551/2519
Collection光电技术研究所被WoS收录文章
Affiliation1.Sichuan Univ, West China Univ Hosp 2, Dept Gynecol & Obstet, Chengdu 610041, Sichuan, Peoples R China
2.Chinese Acad Sci, Inst Opt & Elect, State Key Lab Opt Technol Microfabricat, Chengdu, Sichuan, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Qianying,Duan, Ruiqi,Yuan, Jialing,et al. A reusable localized surface plasmon resonance biosensor for quantitative detection of serum squamous cell carcinoma antigen in cervical cancer patients based on silver nanoparticles array[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2014,9:1097-1104.
APA Zhao, Qianying,Duan, Ruiqi,Yuan, Jialing,Quan, Yi,Yang, Huan,&Xi, Mingrong.(2014).A reusable localized surface plasmon resonance biosensor for quantitative detection of serum squamous cell carcinoma antigen in cervical cancer patients based on silver nanoparticles array.INTERNATIONAL JOURNAL OF NANOMEDICINE,9,1097-1104.
MLA Zhao, Qianying,et al."A reusable localized surface plasmon resonance biosensor for quantitative detection of serum squamous cell carcinoma antigen in cervical cancer patients based on silver nanoparticles array".INTERNATIONAL JOURNAL OF NANOMEDICINE 9(2014):1097-1104.
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