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题名:
A reusable localized surface plasmon resonance biosensor for quantitative detection of serum squamous cell carcinoma antigen in cervical cancer patients based on silver nanoparticles array
作者: Zhao, Qianying1; Duan, Ruiqi1; Yuan, Jialing1; Quan, Yi1; Yang, Huan2; Xi, Mingrong1
刊名: INTERNATIONAL JOURNAL OF NANOMEDICINE
出版日期: 2014
卷号: 9, 页码:1097-1104
关键词: localized surface plasmon resonance ; nanotechnology ; biosensor ; cervical cancer biomarker ; squamous cell carcinoma antigen
文章类型: Article
英文摘要: Squamous cell carcinoma antigen (SCCa), as a tumor biomarker, plays an important role in adjuvant diagnosis, treatment evaluation, and prognosis prediction for cervical cancer patients. Localized surface plasmon resonance (LSPR) technique based on noble metal nanoparticles bypasses the disadvantages of traditional testing strategies, in terms of free-labeling, short assay time, good sensitivity, and selectivity. Herein, we develop a novel and reusable LSPR biosensor for the detection of SCCa. First, a triangle-shaped silver nanoparticle array was fabricated using the nanosphere lithography method. Next, we investigated and verified the feasibility of amino coupling method using 11-mercaptoundecanoic acid (MUA) to form a functionalized chip surface with monoclonal anti-SCCa antibodies on the silver nanoparticles for distinct detection of SCCa. Different concentrations of SCCa were successfully tested in both buffer and human serum by the ultrasensitive and specific LSPR system, with a linear quantitative detection range of 0.1-1,000 pM under optimal conditions. With appropriate regeneration solution, for example 50 mM glycine-HCl (pH 2.0), the LSPR biosensor featured effective fabrication reproducibility, which reduced both production cost and testing time. Our study represents the first application of the LSPR biosensor in cervical cancer, and demonstrates that the rapid, simple, and reusable nanochip can serve as a potential alternative for clinical serological diagnosis of SCCa in cervical cancer patients.
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
研究领域[WOS]: Science & Technology - Other Topics ; Pharmacology & Pharmacy
关键词[WOS]: TUMOR-ANTIGEN ; ASSAY ; RADIOIMMUNOASSAY ; HPV
收录类别: SCI
语种: 英语
WOS记录号: WOS:000331702400001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ioe.ac.cn/handle/181551/2519
Appears in Collections:光电技术研究所被WoS收录文章_期刊论文

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作者单位: 1.Sichuan Univ, West China Univ Hosp 2, Dept Gynecol & Obstet, Chengdu 610041, Sichuan, Peoples R China
2.Chinese Acad Sci, Inst Opt & Elect, State Key Lab Opt Technol Microfabricat, Chengdu, Sichuan, Peoples R China

Recommended Citation:
Zhao, Qianying,Duan, Ruiqi,Yuan, Jialing,et al. A reusable localized surface plasmon resonance biosensor for quantitative detection of serum squamous cell carcinoma antigen in cervical cancer patients based on silver nanoparticles array[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2014,9:1097-1104.
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