We have demonstrated adaptive correction of specimen-induced aberration during in vivo imaging of mouse bone marrow vasculature with confocal fluorescence microscopy. Adaptive optics system was completed with wavefront sensorless correction scheme based on stochastic parallel gradient descent algorithm. Using image sharpness as the optimization metric, aberration correction was performed based upon Zernike polynomial modes. The experimental results revealed the improved signal and resolution leading to a substantially enhanced image contrast with aberration correction. The image quality of vessels at 38- and 75-mu m depth increased three times and two times, respectively. The corrections allowed us to detect clearer bone marrow vasculature structures at greater contrast and improve the signal-to-noise ratio. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
1.Chinese Acad Sci, Key Lab Adapt Opt, Chengdu 610209, Peoples R China 2.Chinese Acad Sci, Inst Opt & Elect, Lab Adapt Opt, Chengdu 610209, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200030, Peoples R China 5.Med X Res Inst, Shanghai 200030, Peoples R China
Wang, Zhibin,Wei, Dan,Wei, Ling,et al. Aberration correction during real time in vivo imaging of bone marrow with sensorless adaptive optics confocal microscope[J]. JOURNAL OF BIOMEDICAL OPTICS,2014,19(8).