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IOE OpenIR  > 光电技术研究所被WoS收录文章  > 期刊论文
题名:
Detection of p53 gene mutation by using a novel biosensor based on localized surface plasmon resonance
作者: Duan, R. Q.1; Yuan, J. L.1; Yang, H.2; Luo, X. G.2; Xi, M. R.1
刊名: NEOPLASMA
出版日期: 2012
卷号: 59, 期号:3, 页码:348-353
关键词: biosensor ; localized surface plasm on resonance ; gene mutation
文章类型: Article
英文摘要: Few studies to date have reported on the development and application of a nanobiosensor based on localized surface plasmon resonance (LSPR) for detecting gene mutations. This study aimed to develop a novel LSPR biosensor used for detecting p53 mutation. Nanosphere lithography was used to fabricate the silver nanoparticles. The DNA probe was designed to recognize the target sequence and immobilized on the chip surface by a covalent-coupling method using amine-group ligands. Synthetic oligonucleotides or PCR products were amplified from genomic DNA taken from blood samples and hybridized with the immobilized probe. Wild-type and mutant p53 was detected by measuring shifts in peak of LSPR extinction spectra. The low detection limit of the sensor for target sequence was 10 nM, and detection occurred over a wide dynamic range (10 nM - 10 mu M). Importantly, the differences in measuring signal between wild-type and mismatched p53 DNA was significant, allowing for this sensor to effectively discriminate against single base mutations. In conclusion, we developed a biosensor with potential as a rapid, label-free, sensitive, and low-cost method for detecting p53 mutation. Our results suggest that such an LSPR-based biosensor provides an attractive alternative for clinical detection of genetic mutation.
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: LABEL-FREE DETECTION ; NANOPARTICLE ARRAY ; OPTICAL BIOSENSOR ; TP53 ; NANOSENSORS ; CANCER ; ASSAY
收录类别: SCI
语种: 英语
WOS记录号: WOS:000301966700015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ioe.ac.cn/handle/181551/2409
Appears in Collections:光电技术研究所被WoS收录文章_期刊论文

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作者单位: 1.Sichuan Univ, Dept Gynecol & Obstet, W China Univ Hosp 2, Chengdu 610041, Peoples R China
2.Chinese Acad Sci, State Key Lab Opt Technol Microfabricat, Inst Opt & Elect, Chengdu 610209, Peoples R China

Recommended Citation:
Duan, R. Q.,Yuan, J. L.,Yang, H.,et al. Detection of p53 gene mutation by using a novel biosensor based on localized surface plasmon resonance[J]. NEOPLASMA,2012,59(3):348-353.
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