IOE OpenIR  > 光电技术研究所被WoS收录文章
Formation and Stimuli-Directed Migration of D. discoideum Slugs in Microchips
Kim, Jinho1; Olsen, Timothy1; Zhuang, Xuye2; Luo, Ji2; Yao, Jun2; Stojanovic, Milan3; Lin, Qiao1
Source PublicationJOURNAL OF MEDICAL AND BIOLOGICAL ENGINEERING
Volume33Issue:3Pages:263-268
2013
Language英语
Indexed BySCI
WOS IDWOS:000322734800004
SubtypeArticle
AbstractThis paper presents a microfluidic device that geometrically constrains the development of individual Dictyostelium discoideum cells into multicellular organisms (slugs). A microchip for the stimuli-directed migration of slugs is also presented. To demonstrate the formation of slugs in a predetermined shape, a microchip is designed to confine the slugs in the vertical direction. In the microchip, sufficient oxygen is supplied to the cells via a membrane, allowing the formation and adaptation of slugs to the shape of the channel In addition, the manipulation of slug migration direction in a microchip via external stimuli, such as light and temperature gradients, that induce phototaxis and thermotaxis of slugs, respectively, is demonstrated. To direct slug migration with external stimuli, an optical fiber is used for phototaxis and an electrical wire heater is used for thermotaxis. Experimental results show slug formation in a predefined geometry in the microchip, suggesting that this chip is potentially useful for understanding the relationship between the shape and function of cells or tissue. The controlled migration of slugs demonstrated in the microchips can potentially be employed in biologically based microactuators or microrobots.
KeywordMicrofluidic Device Microorganism Dictyostelium Discoideum Phototaxis Thermotaxis Bio-microactuator
WOS KeywordDICTYOSTELIUM-DISCOIDEUM ; CULTURE ; ARRAY
WOS Research AreaEngineering
WOS SubjectEngineering, Biomedical
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ioe.ac.cn/handle/181551/1208
Collection光电技术研究所被WoS收录文章
Affiliation1.Columbia Univ, Dept Mech Engn, New York, NY 10027 USA
2.Chinese Acad Sci, Inst Opt & Elect, Chengdu 610209, Peoples R China
3.Columbia Univ, Dept Med, Div Clin Pharmacol & Expt Therapeut, New York, NY 10032 USA
Recommended Citation
GB/T 7714
Kim, Jinho,Olsen, Timothy,Zhuang, Xuye,et al. Formation and Stimuli-Directed Migration of D. discoideum Slugs in Microchips[J]. JOURNAL OF MEDICAL AND BIOLOGICAL ENGINEERING,2013,33(3):263-268.
APA Kim, Jinho.,Olsen, Timothy.,Zhuang, Xuye.,Luo, Ji.,Yao, Jun.,...&Lin, Qiao.(2013).Formation and Stimuli-Directed Migration of D. discoideum Slugs in Microchips.JOURNAL OF MEDICAL AND BIOLOGICAL ENGINEERING,33(3),263-268.
MLA Kim, Jinho,et al."Formation and Stimuli-Directed Migration of D. discoideum Slugs in Microchips".JOURNAL OF MEDICAL AND BIOLOGICAL ENGINEERING 33.3(2013):263-268.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Kim, Jinho]'s Articles
[Olsen, Timothy]'s Articles
[Zhuang, Xuye]'s Articles
Baidu academic
Similar articles in Baidu academic
[Kim, Jinho]'s Articles
[Olsen, Timothy]'s Articles
[Zhuang, Xuye]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Kim, Jinho]'s Articles
[Olsen, Timothy]'s Articles
[Zhuang, Xuye]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.